Tooth shedding evolved over 400 million years ago
The authors of the new study decided to investigate a jaw bone of the 424 million year old fossil fish Andreolepis from Gotland in Sweden, close to the common ancestor of all living bony fish and land vertebrates. The jaw is a tiny thing, less than a centimetre in length, but it hides a wonderful secret: the internal microstructure of the bone is perfectly preserved and contains a record of its growth history. Until recently it has only been possible to see internal structures by physically cutting thin sections from the fossil and viewing them under the microscope, but this destroys the specimen and provides only a two-dimensional image that is hard to interpret.
However, at the European Synchrotron Radiation Facility (ESRF) in Grenoble, France, it is now possible to make tomographic scans that capture the same level of microscopic detail, in three dimensions, without damaging the specimen. Donglei Chen, first author of the study, has spent several years painstakingly ‘dissecting’ the scan data on the computer screen, building up a three-dimensional map of the entire sequence of tooth addition and loss – the first time an early fossil dentition has been analyzed in such detail.
“Every time a tooth was shed, the resorption process created a hollow where it had been attached. When the succeeding replacement tooth was cemented in place by bone of attachment, the old resorption surface remained as a faint buried scar within the bone tissue. I found up to four of these buried resorption surfaces under each tooth, stacked on top of each other like plates in a cupboard. This shows that the teeth were replaced again and again during the life of the fish,” explains Donglei Chen.
This is the earliest known example of tooth shedding by basal resorption, and it seems to be most similar to the process of tooth replacement seen today in primitive bony fish such as gar (Lepisosteus) and bichir (Polypterus). Like in these fish, new replacement teeth developed alongside the old ones, rather than underneath them like in us.
“The amount of biological information we get from the scans is simply astonishing. We can follow the process of growth and resorption right down to cellular level, almost like in a living animal. As we apply this technique to more early vertebrates, we will come to understand their life processes much better – and no doubt we will be in for some major surprises,” says Per Ahlberg, one of the leaders of the project.